Supplementary Figure 2

Quantitation of viral RNA by slot blot hybridization analysis after concentration of virus by centrifugation at 6000 g. Viral supernatant was centrifuged at 6000 g for the time periods indicated. The viral pellet was resuspended in a thirtieth of the original volume. The indicated volumes of (a) unconcentrated supernatant, (b) the resuspended viral pellet, and (c) the post-centrifugation supernatant were loaded onto a nylon membrane in a 48-well slot blot format, hybridized with an enhanced green fluorescent protein probe, and exposed to film. Experiments were repeated three times with similar results.