Supplementary Figure 4

Quantitation of viral RNA by slot blot hybridization analysis after concentration of virus by centrifugation for four hours. Viral supernatant was centrifuged for four hours at the indicated relative centrifugal force (RCF). The viral pellet was resuspended in a hundredth of the original volume. The indicated volumes of (a) unconcentrated supernatant, (b) the resuspended viral pellet, and (c) the post-centrifugation supernatant were loaded onto a nylon membrane in a 48-well slot blot format, hybridized with an enhanced green fluorescent protein probe, and exposed to film. Experiments were repeated three times with similar results.