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Table 1 Viral transduction of fibroblast-like synovial cells

From: Highly efficient genetic transduction of primary human synoviocytes with concentrated retroviral supernatant

FLS Line

Negative control

Unconcentrated supernatant

10X Concentrated supernatant

100X Concentrated supernatant

RA16

0.1

8.2

33.3

25.4

RA25

0.6

23.7

62.2

63.5

RA30

0.0

15.8

40.7

58.2

RA31

0.2

3.7

23.1

45.5

RA32

0.2

19.6

47.3

43.9

Mean ± SD

0.2 ± 0.2%

14.2 ± 8.2%

41.3 ± 14.7%*

47.3 ± 14.8%**

  1. Fibroblast-like synovial cells (FLS) were transduced with pRET2.EGFP retroviral supernatant. The values represent the percentage of enhanced green fluorescent protein-positive cells by flow cytometry. *P < 0.01 compared with unconcentrated supernatant; **P > 0.05 compared with 10X supernatant.
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Arthritis Research & Therapy

ISSN: 1478-6362

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