Supplementary Table 1 PCR primer location and sequence
From: Production of interleukin-1 receptor antagonist by human articular chondrocytes
Primer | Gene | GenBank | Location (bp)* | Sequence 5'-3' |
|---|---|---|---|---|
A | IL-1RN | U65590 | 16,106–16,131 (s) | CAGGTACTGCCCGGGTGCTACTTTAT |
B | IL-1RN | U65590 | 25,827–25,852 (s) | GGCCTCCGCAGTCACCTAATCACTCT |
C | IL-1RN | U65590 | 30,881–30,856 (as) | TACTACTCGTCCTCCTGGAAGTAGAA |
D | IL-1RN | U65590 | 26,130–26,109 (as) | GGTCGCACTATCCACATCTGGG |
E | β-Actin | M10277 | 1559–1583 (s) | CCAAGGCCAACCGCGAGAAGATGAC |
F | β-Actin | M10277 | 2681–2658 (as) | AGGGTACATGGTGGTGCCGCCAGAC |
G | IL-1RN | U65590 | 29134–29115 (as) | TTGACACAGGACAGGCACAT |
H | GAPDH | M32599 | 140–157 (s) | AACGACCCCTTCATTGAC |
I | GAPDH | M32599 | 330–312 (as) | TCCACGACATACTCAGCAC |