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Supplementary Table 2 PCR conditions

From: Production of interleukin-1 receptor antagonist by human articular chondrocytes

cDNA Forward primer Reverse primer Annealing temperature (°C) Product length (bp)
icIL-1Ra1 A C 55 577
sIL-1Ra B C 55 521
Unspliced IL1-Ra B D 55 303
β-Actin E F 60 579
sIL-1Ra (LC) B G 57 268
GAPDH (LC) H I 57 191
  1. LC, Light Cycler™ (Roche Molecular Biochemicals, Basel, Switzerland). GAPDH, glyceraldehyde-3-phosphate dehydrogenase.