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Table 3 Comparison of visual and automated interpretation of IIF ANCA patterns of the 'test set' patients (n = 342) on ethN and formN.

From: Automated interpretation of ANCA patterns - a new approach in the serology of ANCA-associated vasculitis

  ethN Automated interpretation by AKLIDES™
  n (%) cytoplasmic nuclear atypical negative
  cytoplasmic 59 (17.2) 0 (0) 7 (2.0) 22 (6.4)
  nuclear 0 (0) 61 (17.8) 4 (1.2) 0 (0)
  atypical 3 (0.9) 10 (2.9) 40 (11.7) 2 (0.6)
  negative 0 (0) 0 (0) 1 (0.3) 133 (38.9)
Visual interpretation formN  
  n (%) cytoplasmic atypical negative  
  cytoplasmic 91 (26.6) 0 (0) 9 (2.6)  
  atypical 8 (2.3) 0 (0) 0 (0)  
  negative 9 (2.6) 0 (0) 225 (65.8)  
  1. ANCA IIF patterns were first read automatically with AKLIDES™ followed by manual interpretation with a routine fluorescence microscope. Both interpretation methods demonstrated good agreement on ethN and formN (κ = 0.746, 95% CI: 0.667 to 0.825; κ = 0.847, 95% CI: 0.79 to 0,904; respectively). ANCA, antineutrophil cytoplasmic antibody; ethN, ethanol-fixed neutrophils; formN, formalin-fixed neutrophils; IIF, indirect immunofluorescence.