Table 1 Primer sequences for RT-qPCR analysis.
From: Disease severity in K/BxN serum transfer-induced arthritis is not affected by IL-33 deficiency
Target | GenBank | Primer | Primer sequence |
|---|---|---|---|
Il33 | NM_133775.2 | Il33 fwd | 5'-ggtgtggatgggaagaagctg-3' |
Il33 rev | 5'-gaggactttttgtgaaggacg-3' | ||
Il6 | NM_031168.1 | Il6 fwd | 5'-tgaacaacgatgatgcacttgcaga-3' |
Il6 rev | 5'-tctgtatctctctgaaggactctggct-3' | ||
Il1r1 | NM_008362.2 | Il1r1 fwd | 5'-gagttacccgaggtccagtgg-3' |
Il1r1 rev | 5'-gagggctcaggataacagg-3' | ||
Il1rl2 | NM_133193.3 | Il1rl2 fwd | 5'-aaacacctagcaaaagcccag-3' |
Il1rl2 rev | 5'-agactgcccgattttcctatg-3' | ||
Il18r1 | NM_008365.2 | Il18r1 fwd | 5'-ggagatgagggctactactcctgcg-3' |
Il18r1 rev | 5'-gtcttcttgcacattagggtctgagctg-3' | ||
Il18rap | NM_010553 | Il18rap fwd | 5'-cccggaagtgctagaagaca-3' |
Il18rap rev | 5'-acccgcagagcctttttgac-3' | ||
Gapdh | NM_008084.2 | Gapdh fwd | 5'-acggccgcatcttcttgtgca-3' |
Gapdh rev | 5'-aatggcagccctggtgacca-3' |