Presence of miR-125b target sequence in the 3′-UTR of human ADAMTS-4 mRNA and its expression in primary cultured human chondrocytes and human cartilage tissues. (A) TargetScan 6.2 predicted duplex of hsa-miR-125b seed sequence with target sequence in the 3'-UTR of human ADAMTS-4 mRNA. (B) Relative expression of miR-125b, miR125a and miR-4319 in normal chondrocytes (n = 3) and each threshold cycle (Ct) values from real-time PCR. (C) Relative expression of miR-125b in normal and osteoarthritic (OA) chondrocytes and normal, aging and OA cartilage tissue samples (n = 3 for normal chondrocytes normal, aging and OA cartilage from three different donors, n = 7 for OA chondrocytes from seven different donors). The data are expressed as mean and SEM of three independent experiments, each of which was run in triplicate. ** = P <0.001 as measured using an unpaired Student's t test. ADAMTS-4, aggrecanase-1; PCR, polymerase chain reaction; SEM, standard error of the mean; 3'-UTR, 3'-untranslated region.