IL-1β induces ADAMTS-4 expression and suppresses miR-125b expression. (A) Relative expression of miR-125b in normal (n = 3) and osteoarthritic (OA) (n = 7) chondrocytes stimulated with IL-1β for 24 hours. (B) Kinetics of expression of mRNA of ADAMTS-4 and ADAMTS-5 in normal (n = 3) and OA (n = 7) chondrocytes treated with IL-1β for 6, 12 and 24 hours. The data are expressed as mean and SEM of three independent experiments, each of which was run in triplicate. ** = P <0.001 as measured using an unpaired Student's t test. ADAMTS-4, aggrecanase-1; SEM, standard error of the mean.