Figure 3

Modulation of miR-125b affects ADAMTS-4 expression in OA chondrocytes. (A) Relative expression level of miR-125b in human osteoarthritic (OA) chondrocytes transfected with pre-miR-125b, anti-miR-125b or negative control (n = 5). (B) Relative expression level of ADAMTS-4 mRNA 24 hours after IL-1β treatment in normal (n = 3) and OA (n = 5) chondrocytes transfected with pre-miR-125b, anti-miR-125b or negative control. The data are expressed as mean and SEM of three independent experiments, each of which was run in triplicate. ** = P <0.001, * = P <0.05 as measured using an unpaired Student's t test. ADAMTS-4, aggrecanase-1; anti-miR-125b, MiRNA-125b antagomir; pre-miR-125b MiRNA-125b precursor; SEM, standard error of the mean