Figure 5

Restoration of chronic graft vs host disease (Cgvhd)-induced autoantibody (autoAb) by human granulocyte-colony stimulation factor (huG-CSF) correlates with CD4⁺ T cell activation, as well as DC and GR-1⁺ cell expansion. (A) huG-CSF 12 × 2 treatment increased CD69 expression, reduced the percentage of naïve CD62L⁺ CD44^- CD4⁺ T cells and expanded the percentage of the CD62L^- CD44⁺ effector memory CD4⁺ T cells in B6.Sle2c2 mice compared to dextrose-treated controls. (B) huG-CSF 12 × 2 treatment increased the percentage of CD11b⁺ CD11c⁺, GR1^hi CD11b⁺ and GR1^lo CD11b⁺ cells in B6.Sle2c2 mice compared to dextrose-treated controls. Means and standard error of the mean are shown with the significance value from Dunnett's multiple comparison tests between groups with five mice per group (*P < 0.05; **P < 0.01; ***P < 0.001).