Immunoblot analysis of cleavage fragments of Golgi autoantigens (panels b–e) and polyADP-ribose polymerase (PARP, panel a) during apoptotic and necrotic cell death. Jurkat cells were exposed to either 1μM staurosporine (STS) for 2 or 4 hours (left) or 0.1% hydrogen peroxide (H2O2) for 3 hours (right) for the induction of apoptosis and necrosis, respectively. Intact protein and cleavage fragments are indicated. Numbers to left of each blot represent the relative molecular weight (kDa). Note that the 75 kDa band detected by anti-golgin-95 in the control as well as the sample treated with STS for 2 hours may represent an unrelated protein recognized by the antiserum. Also note that this 75 kDa protein was not detected in HEp-2 cells. g160, golgin-160; g97, golgin-97; gm130, golgin-95.