Analysis of immunoglobulin light chain rearrangements in the salivary gland and blood of a patient with Sjögren's syndrome

Table 1 Mutational frequency of Vλ gene rearrangements of B cells from the peripheral blood and from the parotid gland of a patient with Sjögren's syndrome (SS), and of peripheral blood B cells from normal healthy subjects (NHS)

	SS peripheral blood			SS parotid gland			NHS peripheral blood
Vλ	Nonproductive	Productive	P ^*	Nonproductive	Productive	P ^*	Nonproductive	Productive	P ^*
Overall mutational frequency (%)	0.33^†§	0.97^‡	< 0.001	0.76^†	3.32^‡	< 0.001	0.60^§	1.12	< 0.001
Mutated Vλ gene rearrangements (n)	14	30		5	24		27	100
Total Vλ gene rearrangements (n)	27	54		9	29		55	172
% mutated Vλ gene rearrangements	52	56		55	83		49	58

^* Significant difference between the mutational frequency found in the nonproductive versus the productive Vλ gene repertoire (χ² test). ^†P < 0.01 and ^‡P < 0.001, significant difference between the mutational frequency of the nonproductive and productive, respectively, Vλ gene rearrangements of B cells from the peripheral blood and the parotid gland of a patient with SS. ^§P < 0.05, significant difference between the mutational frequency of the nonproductive Vλ gene rearrangements of peripheral blood B cells from the patient with SS and of normal donors.

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