Analysis of immunoglobulin light chain rearrangements in the salivary gland and blood of a patient with Sjögren's syndrome

Table 2 Mutational frequency of Vκ gene rearrangements of B cells from the peripheral blood and from the parotid gland of a patient with Sjögren's syndrome (SS), and of peripheral blood B cells from normal healthy subjects (NHS)

	SS peripheral blood			SS parotid gland			NHS
Vκ	Nonproductive	Productive	P ^*	Nonproductive	Productive	P ^*	Nonproductive	Productive	P ^*
Mutational frequency (%)	0.20^§	0.77^†‡	< 0.001	0.09	2.35^†	< 0.001	0.48^§	1.08^‡	< 0.001
Mutated Vκ gene rearrangements (n)	15	21		4	43		55	133
Total Vκ gene rearrangements (n)	40	39		23	50		232	321
% mutated Vκ gene rearrangements	38	54		17	86		24	41

^* Significant difference between the mutational frequency found in the nonproductive versus the productive Vκ gene repertoire (χ² test). ^†P = 0.005 and ^‡P < 0.001, significant differences in the mutational frequency of the productive Vκ gene rearrangements of B cells from the peripheral blood compared with the parotid gland of a patient with SS or with normal donors. ^§P < 0.001, significant difference between the mutational frequency of the nonproductive Vκ gene rearrangements of peripheral blood B cells from the patient with SS and of normal donors.

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