Figure 4

Quantitative analysis of proteoglycans and the aggrecan neoepitope CS846. (A) Proteoglycans were quantitatively assessed in fresh and cultured (+/-TGF-β1) cartilage, as well as in the culture supernatant by the measurement of GAG by the DMB assay; (B) quantitative analysis of the aggrecan neoepitope CS846 was performed by a specific ELISA assay. The values of the content in cartilage (blue bars) and the cumulative release of GAG/CS846 into the culture medium (red lines) are expressed as means ± SEM. BNC, bacterial nana-cellulose; DMB, dimethylene blue; GAG, glycosaminoglycan; SEM, standard error of the mean; TGF-β1, transforming growth factor-β1.