Figure 4

Percentage of immature/transitional IL-10-producing B peripheral-cell subtype in patients with primary Sjögren's syndrome (pSS). CD19⁺ blood B cells were obtained by positive selection with microbeads. (A) Representative contour plot of CD19⁺ B cells. An electronic gate was made for CD38^hi cells. (B) From the gate A CD19⁺⁄CD38^hi⁄CD24^hi cells were determined. (C) From the gate B CD19⁺⁄CD38^hi⁄CD24^hi⁄CD20⁺ were defined. (D) From the latter CD19⁺⁄CD38^hi⁄CD24^hi⁄CD20⁺⁄IL-10⁺ cells were determined. Bar graphs show percentage of (E) CD19⁺⁄CD38^hi⁄CD24^hi⁄CD5⁺⁄IL-10⁺, (F) CD19⁺⁄CD38^hi⁄CD24^hi⁄CD10⁺⁄IL-10⁺, (G) CD19⁺⁄CD38^hi⁄CD24^hi⁄CD20⁺⁄IL-10⁺, (H) CD19⁺⁄CD38^hi⁄CD24^hi⁄CD27^-⁄IL-10⁺, (I) CD19⁺⁄CD38^hi⁄CD24^hi⁄CXCR4⁺⁄IL-10⁺-, and (J) CD19⁺⁄CD38^hi⁄CD24^hi⁄CXCR7⁺⁄IL-10⁺-producing B peripheral cells. A total of 50,000 to 100,000 events were recorded for each sample before any gate setting and were analyzed with the CellQuestPro software (BD Biosciences). (E-J) Results are expressed as median, 10^th, 25^th, 75^th, and 90^th percentiles. *P < 0.05.