Figure 5

Percentage of indoleamine 2,3-dioxygenase (IDO)- and Foxp3-expressing peripheral blood cells in patients with primary Sjögren's syndrome (pSS). (A,B) Control of fluorescein isothiocyanate (FITC)-labeled-rabbit anti-sheep specificity staining. (C) An electronic gate was made for CCR6⁺ cells. (D) From the gate C CCR6⁺⁄CD123^hi cells were determined. (E) From the latter CCR6⁺⁄CD123^hi⁄IDO⁺ cells were defined. (F) An electronic gate was made for CD4⁺ cells. (G) From the gate F CD4⁺⁄CD25^hi were determined and an electronic gate was made for double positive cells. (H) From the latter CD4⁺⁄CD25^hi⁄Foxp3⁺ cells were defined. Bar graphs show percentage of (I) CCR6⁺⁄CD123^hi⁄IDO⁺ cells, (J) CD4⁺⁄CD25^hi⁄Foxp3⁺ cells, (K) CD8⁺⁄CD28^-⁄Foxp3⁺ cells. A total of 100,000 to 250,000 events were recorded for each sample before any gate setting, and were analyzed with the CellQuestPro software (BD Biosciences). (I-K) Results are expressed as median, 10^th, 25^th, 75^th, and 90^th percentiles. *P < 0.05.