Figure 8

Colchicine modulates MSU-induced IL-8 secretion in human neutrophils. Neutrophils were incubated in colchicine (10 µM) (+) or DMSO (-) for 30 min at 37°C prior to adding MSU (1 mg/ml) or buffer to the neutrophil-colchicine mixture and incubating it for a further 3 h at 37°C. The quantity of IL-8 in the supernatant was determined by ELISA. A compilation of the data from three independent experiments is shown (n = 3).