Versican V0 and CCL2 protein levels in monocyte culture supernatants. Versican V0 and CCL2 protein levels in monocyte culture supernatants derived from systemic sclerosis (SSc) patients and healthy controls. (A) Representative immunoblot evaluating versican V0 protein expression. Monocyte culture supernatants were concentrated, truncated, and applied to immunoblots. (B) Versican V0 protein levels in monocytes from 14 patients with SSc and 11 healthy controls, semiquantitatively measured by densitometry. (C) CCL2 protein levels in monocytes from 16 patients with SSc and 13 healthy controls. CCL concentration in culture supernatants was measured by an ELISA. Each bar in the graph denotes the mean. Results from the two groups were compared by Mann-Whitney U test.