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Table 1 Description of the LNA probe and primer sequences used to quantify gene expression

From: Natriuretic peptide receptors regulate cytoprotective effects in a human ex vivo 3D/bioreactor model

Gene Gene ID Sequences Product length
Npr2 4882 Probe: 5'-FAM-ATCGCTGGCTGCTTCTATG-BHQ1-3'
Sense: 5'-CCCTTCCCTGATGAACCT -3'
Antisense: 5'-CCCTGCATCTTTTCCACAA -3'
132
Npr3 4883 Probe: 5'-FAM-TTCTCTCCAAAGGTTCCCG-BHQ1-3'
Sense: 5' TTTAAGAAAAACTAGTGACATTGGA-3'
Antisense: 5'- CCACCCTTCCTCTTTCCT-3'
146
CNP 4880 Probe: 5-'FAM-CGCCAATCTCAAGGGC-BHQ1-3'
Sense: 5'-TCAGAAGAAGGGCGACAA -3'
Antisense: 5'-TGGCTCCTTTGTATTTGCG -3'
158
GAPDH 2597 Probe: 5'-HEX-CAGTCAGCCGCATCTTCT-BHQ1-3'
Sense: 5'-TCTCTGCTCCTCCTGTTC-3'
Antisense: 5'-CGCCCAATACGACCAAAT-3'
160
Aggrecan 176 Probe: 5-'FAM-CCAACTCTTCAAGGTGA-BHQ1-3'
Sense: 5'-GACTGAAGTTCTTGGAGAA-3'
Antisense: 5'-CACGAAAACCCAGAGTAA-3'
109
Collagen type II 1280 Probe: 5'-FAM-TCTGTCTCCTTGCTTGCCA-BHQ1-3'
Sense: 5'-GGAGTCAAGGGTGATCGT-3'
Antisense: 5'-CTTGTGCACCAGCTTCTC-3'
200
  1. Primers used in PCR experiments with locked nucleic acid (LNA) probes produced amplicons between 132 and 160 base pairs. Probes contain fluorescein (FAM) or 6-carboxyhexafluorescein (HEX) as the 5'-reporter dye and Black Hole Quencher 1 (BHQ1) as the 3'-quencher. Nucleotides highlighted in bold denote the LNA base.