Western blot and densitometry analysis. (A) Western blot analyses at day 28 post transduction for in vitro expression of collagen (COL) I, COL II and COL X. (B) Densitometric analysis illustrating COL/glyceraldehyde-3-phosphate dehydrogenase (GAPDH) expression ratio (Phoretix 1D software; TotalLab Ltd, Newcastle, UK). Adipose-derived stem cells (ASCs) transduced with Ad.IGF-1/Ad.FGF-2 (multiplicity of infection 50 for each vector) showed almost threefold increased expression of COL II compared with the positive control. Similar low expressions of COL × were observed in adenoviral transduced ASCs and the positive control. Expression of COL I was undetected in the experimental groups. FGF-2, fibroblast growth factor-2; IGF-1, insulin-like growth factor-1; WB, positive control for type I collagen from cultured osteoblasts.