Figure 3
Expression of focal adhesion molecules in dermal blood microvascular endothelial cells and lymphatic microvascular endothelial cells. Representative immunofluorescent images of focal adhesion molecules in blood microvascular endothelial cells (B-MVECs) (A) and lymphatic microvascular endothelial cells (Ly-MVECs) (B) cultured with healthy sera are shown. Representative immunofluorescent photomicrographs show double-labeling for αvβ3 integrin (green) (a), phosphorylated focal adhesion kinase (FAK) (red) (b) and a merged image (c). Representative immunofluorescent photomicrographs show double-labeling for phalloidin (green) (d), vinculin (red) (e) and a merged image (f). 4′,6-diamidino-2-phenylindole (DAPI)-stained nuclei are blue in merged images. In both B-MVECs and Ly-MVECs, αvβ3 integrin (a) and phosphorylated FAK (b) are co-localized. αvβ3 integrin (a), phosphorylated FAK (b) and vinculin (e) are visible as small dots distributed all over the cell surface. Phalloidin-stained actin filaments (d) are arranged in long strands running parallel to cell borders. Original magnification, ×40.