Transcriptomic signatures in cartilage ageing

Table 1 Gene primer sequences used in RNA-Seq validation

Gene	Accession code	Primer sequence
GAPDH^a	AF157626	F: GCATCGTGGAGGGACTCA
		R: GCCACATCTTCCCAGAGG
TBP^a	XM_001502211	F: TGCTGCTGTAATCATGAGGGTAA
		R: TCCCGTGCACACCATTTTC
ACTB^a	AF035774	F: CCAGCACGATGAAGATCAAG
		R: GTGGACAATGAGGCCAGAAT
18S^a	AJ311673	F: GGCGTCCCCCAACTTCTTA
		R: GGGCATCACAGACCTGTTATTG
RUNX2	XM_001502519	F: TCCCTGAACTCTGCACCAAG
		R: GCCAGGTAGGAGGGGTAAGA
IL7R	NM_001081942	F: GGCTATGCACAGAATGGAGACT
		R: CAACTGGCTGTAGCACGAGA
SRPX	XM_001489643	F: CTGAGAACAAGGGCG-TTGC
		R: CCGGAGCGTTGAGTTTGC
ACSL5	XM_001915998	F: CCTGGGCTCCTATCTCTTGC
		R: CGGAGATGATCCACTCTGGC
DKK	NM_001267802	F: TAGAACCCTGGGACCTCTGG
		R: GTGTCACTTTGCAAGCCTGG
ADAMTS4^b	NM_001111299	F: CAGCCTGGCTCCTTCAAAAA
		R: CCGCAGGAATAGTGACCACAT
COL1A1^a	O46388	F: GACTGGCAACCTCAAGAAGG
		R: CAATATCCAAGGGAGCCACA
COL2A1^a	NM_001081764	F: TCAAGTCCCTCAACAACCAGAT
		R: GTCAATCCAGTAGTCTCCGCTCTT
COL10A1^a	XM_001504101	F: TGCCCAGTGGACAGGTTTCT
		R: GTCTTTTCGTTTCTAGTCAGATTTTGAA
MMP1^b	NM_001081847	F: GGTGAAGGAAGGTCAAGTTCTGAT
		R: AGTCTTCTACTTTGGAAAAGAGCTTCTCT
MMP13^b	NM_001081804	F: CTGGAGCTGGGCACCTACTG
		R: ATTTGCCTGAGTCATTATGAACAAGAT
IL1β^b	NM_001082526	F: GAGCCCAATCTTCAACATCTATGG
		R: CAGGCTTGGTAAAAGGACTTGGTAT
TNFα^b	NM_001081819	F: GCTCCAGACGGTGCTTGTG
		R: GCCGATCACCCCAAAGTG
TGFβ^b	NM_001081849	F: CCCTGCCCCTACATTTGGA
		R: CGGGTTGTGCTGGTTGTACA

ACSL5, acyl-CoA synthetase long-chain family member 5; ACTB, beta-actin; ADAMTS4, a disintegrin and metalloproteinase with thrombospondin motifs 4; COL1A1, collagen type I, alpha 1; COL2A1, collagen type II, alpha 1; COL10A1, collagen type X, alpha 1; DKK1, dickkopf homolog 1; F, forward; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; IL-1β, interleukin 1 beta; IL7R, interleukin 7 receptor; MMP1, matrix metalloproteinase 1; MMP-13, matrix metalloproteinase 13; R, receptor; RUNX2, Runt-related transcription factor 2; 18S, 18 ribosomal RNS; SRPX, Sushi repeat-containing protein; TBP, TATA box binding protein; TGFβ, transforming growth factor beta; TNFα, tumour necrosis factor alpha;. ^aPrimer pairs previously published in [29]. ^bPrimer pairs previously published in [28].

ISSN: 1478-6362