Table 2 Primers used for quantitative real-time PCR validation.
From: Genomic characterization of remission in juvenile idiopathic arthritis
Gene symbol | Primer direction1 | Primer sequence (5' ~ 3') |
|---|---|---|
BIRC3 | F | CATGGGTTCAACATGCCAAGTGGT |
BIRC3 | R | TTCATCTCCTGGGCTGTCTGATGT |
C3 | F | GTGGAAATCCGAGCCGTTCTCT |
C3 | R | GATGGTTACGGTCTGCTGGTGA |
CCR2 | F | AGTTCAGAAGGTATCTCTCGGTG |
CCR2 | R | GGCGTGTTTGTTGAAGTCACT |
CCR6 | F | CTGAACCCTGTGCTCTACGCTT |
CCR6 | R | CACAGGAGAAGCCTGAGGACTT |
CD22 | F | GCGCAGCTTGTAATAGTTGGTGC |
CD22 | R | CACATTGGAGGCTGACCGAGTT |
CXCR6 | F | CAGTTCAGCAAGGTCTTTCTGCC |
CXCR6 | R | AGGTTCACCAGGAACACATCCG |
EFR3A | F | ATTCAGTGCCATGTGCCATTCCTG |
EFR3A | R | GCCCGAAGTTCATCGTTGACTGTT |
FOXO1 | F | CTACGAGTGGATGGTCAAGAGC |
FOXO1 | R | CCAGTTCCTTCATTCTGCACACG |
FUZ | F | AGCCAGTTGTATCCACAGCTTCTG |
FUZ | R | CCGAGGATGTCTGTGTGAAGGG |
GM2A | F | TCGTTCCTGGAAATGTGACCCTCA |
GM2A | R | CAGCTGCCAATGTAGTCTGTGCAT |
GZMA | F | CCACACGCGAAGGTGACCTTAA |
GZMA | R | CCTGCAACTTGGCACATGGTTC |
IER5 | F | ACCTCATCAGCATCTTCGGTTCCA |
IER5 | R | TTCATGTCTCTCAGCACCGGCTTA |
IL6ST | F | CACCCTGTATCACAGACTGGCA |
IL6ST | R | TTCAGGGCTTCCTGGTCCATCA |
KLRD1 | F | GAGCCAGCATTTACTCCAGGAC |
KLRD1 | R | GCACAGAGATGCCGACTTTCGT |
KMO | F | ATACTCGAGTGGCTACCTTCACAC |
KMO | R | TCTGATCTTCCAGGCCAACAGCTT |
NBN | F | TCTGTCAGGACGGCAGGAAAGA |
NBN | R | CACCTCCAAAGACAACTGCGGA |
NSMAF | F | GTCTGAACACCTTCACGAGTGG |
NSMAF | R | CTGTTCAAGTCTACACCTCCTTC |
RNF167 | F | ATGGGTCAGTCTTTATTGCGCTGC |
RNF167 | R | ATCCAGCCTTCTGGGCATTTAGGA |
STAT1 | F | ATGGCAGTCTGGCGGCTGAATT |
STAT1 | R | CCAAACCAGGCTGGCACAATTG |
TARP | F | CCCCAAGCCCACTATTTTTC |
TARP | R | TGTTGCTCTTCTTTTCTTGCC |
TNFAIP6 | F | TCACCTACGCAGAAGCTAAGGC |
TNFAIP6 | R | TCCAACTCTGCCCTTAGCCATC |
TRAF3 | F | AGCAGAGGTTGTGCAGAGCAGTTA |
TRAF3 | R | TCATCGGAACCTGACTCTTGCAGT |
TRGV5 | F | TGACTCAGGAAGACCAGCTC |
TRGV5 | R | TCTTAAAACTCCGGCCCCAC |
TRIM4 | F | ACGCCACACAGTGGAAGGATAAGA |
TRIM4 | R | TCTTCAACCAGGAAGTTGTGCAGC |
TST | F | TTCCAGCTGGTGGATTCAAGGTCT |
TST | R | AGAGATCCACCTTCTTGGTCTGGA |
UBTF | F | ATGGATTCATAAGGCCCTGGAGCA |
UBTF | R | TTTGTCCGAGAGCTGAGACCACT |
XIAP | F | TGGCAGATTATGAAGCACGGATC |
XIAP | R | AGTTAGCCCTCCTCCACAGTGA |
ZNF277 | F | AATGCAGGAAGACCGTGATGGGA |
ZNF277 | R | AGCGGCTCCAGGATACAATCCTTA |