Figure 4

NAC previously primed with DexVD3 DC secrete IL-8 during the rest phase. Cytokine and chemokine profiling was performed on culture supernatants from NAC previously primed with various DC populations generated from pSS patients. The cell-free culture supernatants were collected after co-culture of DC and NAC and stored in aliquots at -20°C until analysis. The amount of cytokines/chemokines secreted was analyzed using a 25-plex bead assay and the concentrations are given in pg/ml. The bars show the median, n = 6. The differences between the secreted amounts of cytokines are marked with the following: *P ≤ 0.05; **P ≤ 0.01. Cytokines and chemokines that showed results below the detection limit (IL-10, Eotaxin, IL-17, GM-CSF, IL-1β, IL-4) and those that where the differences were not significant are not shown. The DC population that was used to stimulate NAC is shown on the x-axis. See Figure 1 for definitions. GM-CSF, granulocyte-macrophage colony-stimulating factor; NAC, nonadherent cells.