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Table 1 Values of mitochondrial respiratory chain complexes in cultures of normal chondrocytes treated with two NO donor compounds

From: Nitric oxide compounds have different effects profiles on human articular chondrocyte metabolism

  Normal
chondrocytes
Cells treated with
1 mM NOC-12
Cells treated with
1 mM SNP
Age, years 49.8 ± 21.2 (14) 44.2 ± 19.4 (5) 59.7 ± 18.9 (11)
Proteins, mg/ml 3.1 ± 0.9 (14) 2.8 ± 1.4 (5) 4.2 ± 1.4 (11)
CS enzymatic activity (nmol/min/mg protein) 108.9 ± 38.5 (14) 146.9 ± 54.7 (5) 106.6 ± 26.2 (11)
Mitochondrial complex activitya    
   Complex I 30.1 ± 13.4 (14) 6.8 ± 2.3 (5)* 22.8 ± 19.1 (11)
   Complex II 7.4 ± 2.3 (14) 6.8 ± 1.6 (5) 10.2 ± 1.81 (11)
   Complex III 53.5 ± 18.4 (14) 35.2 ± 9.3 (5)* 46.3 ± 9.7 (11)
   Complex IV 61.2 ± 8.3 (14) 15.7 ± 3.1 (5)* 40.2 ± 11.3 (11)*
  1. Values are the mean ± SD (number). *P ≤ 0.05 versus untreated chondrocytes. aCS-corrected complex activity is expressed as (nmol/minute/mg protein)/(CS-specific activity) × 100. NO, nitric oxide; NOC-12, N-ethyl-2(1-ethyl-2 hydroxy-2-nitrosohydrazine; CS, citrate synthase; Complex I, rotenone-sensitive NADH-coenzime Q1 reductase; complex II, succinate dehydrogenase; complex III, antimycin-sensitive ubiquinol cytochrome c reductase; complex IV, cytochrome c oxidase. Chondrocytes were treated with NOC-12 for 24 hours and SNP for 5 hours.