Immunostaining for α-SMA (arrows, myofibroblasts nuclei) incutaneous samples. Representative tissue sample from: (A) Sham animal; (B) HOCl mice; (C) HOCl + PTU animal(Original magnification, ×40). The arrows show strong diffusestaining of myofibroblasts nuclei (dark brown staining); (D) Number of myofibroblasts from the three experimental groups(HOCl + PTU group, n = 10; HOCl group, n = 10;Sham, n = 5). The increase of myofibroblast population inthe skin of HOCl mice is prevented by propylthiouraciladministration. Values are expressed as the mean and SD. *P < 0.001 versus Sham; #P < 0.001 versusHOCl.