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Figure 2 | Arthritis Research & Therapy

Figure 2

From: An organ culture system to model early degenerative changes of the intervertebral disc II: profiling global gene expression changes

Figure 2

(A-B) Diagrams illustrating genes with the highest upregulation (A) and downregulation (B) in expression between the experimental (Exp.) and control (Ctr.) discs. (C-E) Quantitative RT-PCR confirms the changes in gene expression identified by microarray analysis between the Ctr. and Exp. discs. Quantitative PCR expression levels were normalized to hypoxanthine phosphoribosyltransferase 1 (Hprt1) and shown relative to the Ctr., N = 3 independent experiments. (F) Western blot analysis of rat nucleus pulposus (NP) tissue lysates shows an increase in expression of Syndecan-4 (SDC4) and a decrease in expression of Keratin 19 (KRT19) in the experimental group, N = 3 independent experiments.

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Arthritis Research & Therapy

ISSN: 1478-6362

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