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Figure 1 | Arthritis Research & Therapy

Figure 1

From: Dynamic pressurization induces transition of notochordal cells to a mature phenotype while retaining production of important patterning ligands from development

Figure 1

Schematic of the tissue isolation and loading protocol. Pig spines were dissected aseptically and the notochordal cell (NC)-rich nucleus pulposus (NP) tissue was isolated and preconditioned for 4 days in 1% oxygen, 5% carbon dioxide at 37°C in Dulbecco's modified Eagle's medium with 1× insulin transferrin and selenium. NP tissue was divided into three groups (Control group, 1 Dose pressurization group, Daily pressurization group) and placed in airtight Whirl-Pak bags (Nasco, Thermo Fisher Scientific, Waltham, MA, USA) for loading. The Control group was placed in a 37°C water bath for 2 hours every day for 8 days. The 1 Dose group was placed in a custom hydrostatic pressure chamber attached to an Instron 8511 servo hydraulic tension/compression system (Instron, Norwood, MA, USA) for 2 hours on 1 day with 0.5 to 2 MPa load at 0.1 Hz and then in the water bath for 2 hours each day thereafter [38]. The Daily pressurization group was placed in the chamber for 2 hours each day for 8 days with same loading regime as the 1 Dose group.

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