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Figure 2 | Arthritis Research & Therapy

Figure 2

From: Dynamic pressurization induces transition of notochordal cells to a mature phenotype while retaining production of important patterning ligands from development

Figure 2

Quantification of large notochordal cells versus small nucleus pulposus cells. The number of notochordal cells (NCs) and small nucleus pulposus cells (SNPCs) in a total of nine images (3 images×3 spines used = 9) from each group (Control, 1 Dose and Daily) were counted and the percentage of NCs and percentage of SNPCs were calculated from the total number of cells in each image. (A) Control group image. (B) Pressurization group image. Black arrows, NCs; blue arrows, SNPCs. A large NC was defined as nuclei in direct contact and surrounded by large vacuoles, and an SNPC was defined as nuclei surrounded by matrix with no contact with vacuoles. V, vacuoles (green arrows). Scale bar = 50 μm.

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