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Figure 3 | Arthritis Research & Therapy

Figure 3

From: Dynamic pressurization induces transition of notochordal cells to a mature phenotype while retaining production of important patterning ligands from development

Figure 3

Cell viability and metabolic activity in nucleus pulposus tissue with and without pressurization. (A) Viability was assessed using calcein (green = live) and ethidium (red = dead) staining and showed no differences between groups, with the majority of the cells stained green indicating high viability (images captured on a fluorescent inverted microscope, bar =10 μm). (B) Metabolic activity was significantly increased for the Daily pressurization group (P < 0.05) as assessed by the (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay compared with unloaded control and 1 Dose load groups.

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Arthritis Research & Therapy

ISSN: 1478-6362

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