Figure 6From: Dynamic pressurization induces transition of notochordal cells to a mature phenotype while retaining production of important patterning ligands from developmentImmunohistochemistry for Sonic Hedgehog and Semaphorin 3A expression. Sonic Hedgehog (SHH) positive staining in nucleus pulposus (NP) tissue and a qualitative increase was observed with pressurization associated both with the cell and matrix (A). Semaphorin 3A (Sema3A) expression was also detected in NP tissue in the cells and matrix, but no differences in expression were observed with load (B).Back to article page