Prophylactic administration of sclerostin antibody to CIA mice does not prevent focal bone erosion in periarticular bone in the ankle joint. (A, B, C) Bar plots represent mean (and standard deviation of) smoothness parameter (BSsmooth/BS), ratio between bone surface and bone volume (BS/BV), representing surface density, and ankle joint bone volume (BV), respectively. (D) Representative three-dimensional renditions of the hind paws scanned by micro computed tomography. (E) Representative longitudinal mid-sections on the ankle joint and all the bones in the tarsus. Bone volume and erosion parameters were evaluated in the region of interest displayed on the panel A (dotted selection on control (CTR)). The one-way ANOVA test with Tukey's post hoc comparison was used for statistical evaluation of all parameters shown in the bar plots. *P < 0.05 for the difference versus CTR; #P < 0.05 for the difference versus collagen-induced arthritis (CIA) + PBS.