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Table 3 RT-PCR primers sequences (1st BASE Pte. Ltd., Singapore)

From: The effects of staged intra-articular injection of cultured autologous mesenchymal stromal cells on the repair of damaged cartilage: a pilot study in caprine model

Gene

RT-PCR primer sequences

Tm (°C)

GC (%)

Collagen II (Forward)

5′-CTG GAT GCC ATG AAG GTT TT-3′

58.4

45.0

Collagen II (Reverse)

5′-TCT TGT CCT TGC TCT TGC TG-3′

60.4

50.0

Aggrecan (Forward)

5′-GCA AGT GGT CTT CCT TCT GG-3′

62.4

55.0

Aggrecan (Reverse)

5′-TTC CAC CAA TGT CGT ATC CA-3′

58.4

45.0

Sox 9 (Forward)

5′-TGA AGA AGG AGA GCG AGG AG-3′

62.4

55.0

Sox 9 (Reverse)

5′-GAC GTG CGG CTT GTT CTT-3′

59.9

55.6

GAPDH (Forward)

5′-GCT CTC TTC CAG CCT TCC TT-3′

62.4

55.0

GAPDH (Reverse)

5′-TAG AGG TCC TTG CGG ATG TC-3′

62.4

55.0

  1. GC (%), GC content; Tm (°C), Melting temperature.