Gene | RT-PCR primer sequences | Tm (°C) | GC (%) |
---|---|---|---|
Collagen II (Forward) | 5′-CTG GAT GCC ATG AAG GTT TT-3′ | 58.4 | 45.0 |
Collagen II (Reverse) | 5′-TCT TGT CCT TGC TCT TGC TG-3′ | 60.4 | 50.0 |
Aggrecan (Forward) | 5′-GCA AGT GGT CTT CCT TCT GG-3′ | 62.4 | 55.0 |
Aggrecan (Reverse) | 5′-TTC CAC CAA TGT CGT ATC CA-3′ | 58.4 | 45.0 |
Sox 9 (Forward) | 5′-TGA AGA AGG AGA GCG AGG AG-3′ | 62.4 | 55.0 |
Sox 9 (Reverse) | 5′-GAC GTG CGG CTT GTT CTT-3′ | 59.9 | 55.6 |
GAPDH (Forward) | 5′-GCT CTC TTC CAG CCT TCC TT-3′ | 62.4 | 55.0 |
GAPDH (Reverse) | 5′-TAG AGG TCC TTG CGG ATG TC-3′ | 62.4 | 55.0 |