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Table 1 Peptide sets immobilized on the microarrays for surface plasmon resonance imaging analysis a

From: ACPA fine-specificity profiles in early rheumatoid arthritis patients do not correlate with clinical features at baseline or with disease progression

Set

 

Origin

Peptide sequence

Position of citrulline residuesb

1

A

α-enolase (cyclic)

CKIHAR EIFDSR GNPTVECZO

 
 

B

 

CKIHAX EIFDSX GNPTVECZO

9, 15

2

A

β-actin

MKILTER GYSFTTAER EIVRDIKEKLZO

 
 

B

 

MKILTEX GYSFTTAEX EIVXDIKEKLZO

196, 206, 210

3

A

CCP2-like (cyclic)

c

 
 

B

  

n.r.

4

A

Collagen type II

GLPGVKGHR GYPGLDGAZO

 
 

B

 

GLPGVKGHX GYPGLDGAZO

290

5

A

Fibrinogen

FLAEGGGVR GPRVVERHZO

 
 

B

 

FLAEGGGVX GPRVVERHZO

α35d

6

A

Fibrinogen

TSSTSYNR GDSTFZO

 
 

B

 

TSSTSYNX GDSTFZO

α591d

7

A

Fibrinogen

NEEGFFSAR GHRPLDKKZO

 
 

B

 

NEEGFFSAX GHRPLDKKZO

β44e

8

A

Fibrinogen

EEAPSLR PAPPPISGGGYR AR PAKAAAZO

 
 

B

 

EEAPSLX PAPPPISGGGYX AX PAKAAAZO

β60, 72, 74e

9

A

Fibronectin

LTVGLTRR GQPRQYZO

 
 

B

 

LTVGLTXX GQPRQYZO

1,035, 1,036

10

A

Fibronectin

YNQYSQR YHQRTNZO

 
 

B

 

YNQYSQX YHQRTNZO

2356

11

A

Filaggrin (cfc1; cyclic)

HQCESTR GRSRGRCGRSGSZO

f

 

B

 

HQCESTX GRSRGRCGRSGSZO

 

12

A

MNDA

KLTSEAR GR IPVAQKZO

 
 

B

 

KLTSEAX GX IPVAQKZO

127, 129

13

A

Peptide library

c

 
 

B

  

n.r.

14

A

Peptide library

c

 
 

B

  

n.r.

15

A

Peptide library

c

 
 

B

  

n.r.

16

A

Peptide library

c

 
 

B

  

n.r.

17

A

Vimentin

MSTR SVSSSSYRR MFGGPZO

 
 

B

 

MSTX SVSSSSYXX MFGGPZO

4, 12, 13

18

A

Vimentin

GVYATR SSAVR LR SSVPGZO

 
 

B

 

GVYATX SSAVX LX SSVPGZO

64, 69, 71

19

A

Vimentin

LTAALR DVR QQYESZO

 
 

B

 

LTAALX DVX QQYESZO

270, 273

20

A

Vimentin

SLNLR ETNLDSLPLVDZO

 
 

B

 

SLNLX ETNLDSLPLVDZO

424

  1. aCCP2, cyclic citrullinated peptide 2; MNDA, myeloid cell nuclear differentiation antigen; n.r., not relevant; O, biotinylated lysine; X, citrulline; Z, aminohexanoic acid. bAmino acid position in the corresponding polypeptide relative to the N-terminal methionine of the primary translation product. cSequence is defined in patent EP2071335. dα-Chain of fibrinogen. eβ-Chain of fibrinogen. fDerived from sequence presented in [27].