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Table 2 Presence of mRNA for selected glycosyltransferases in OA chondrocytes

From: Glycophenotyping of osteoarthritic cartilage and chondrocytes by RT-qPCR, mass spectrometry, histochemistry with plant/human lectins and lectin localization with a glycoprotein

MAN1C1 0.8 MGAT4A 172.6 ST6Gal1 676.8
MAN2A1 449.9 MGAT4B 77.0 ST6Gal2 5.2
MGAT1 723.3 MGAT5A 387.3 ST3Gal3 59.4
MGAT2 2,328.2 MGAT5B 2.9 ST3Gal4 153.7
MGAT3 10.1 FUT8 263.9 ST3Gal6 14.7
   B4GALNT3 104.4   
    O-glycans      Chondrocyte markers  
GALNT 3,925.3 ST3Gal1 60.4 AGC 1,704.7
B3GNT 141.8 ST3Gal2 75.7 COL2 9,953.7
GCNT1 375.5 ST6GalNAc1 n.d. COL1 5,722.9
FUT1 0.4 ST6GalNAc2 n.d.   
3OST 19.4 ST6GalNAc3 0.7   
   ST6GalNAc4 9.8   
  1. Distinct mRNA species were quantified using RT-qPCR. Numbers denote relative copy numbers with respect to the expression of the GAPDH gene arbitrarily set at 1,000. cDNA of OA chondrocytes from five donors was pooled and analyzed in duplicate. All series of measurements (technical replicates) had a standard deviation below 1.5%.
  2. MAN2A1 and MGAT2: conversion of oligomannosides to complex-type N-glycans. MGAT1: committing step for synthesis of hybrid- and complex-type N-glycans. MAN1C1: trimming of oligomannosidic structures. MGAT3: introduction of bisecting GlcNAc to the core of complex-type glycans in β1,4-linkage. MGAT4 and MGAT5: production of tri- and tetra-antennary N-linked sugar chains. FUT8: transfer of fucose to the core of complex-type glycans in α1,6-linkage. B4GALNT3: β1,4-N-acetylgalactosaminyltransferase 3, forming the LacdiNAc terminus. ST6GAL1, ST6GAL2: N-glycan α2,6-sialyltransferases. ST3GAL3, ST3GAL4, ST3GAL6: N-glycan α2,3-sialyltransferases. GALNT1: initiates O-linked mucin-type glycosylation in the Golgi apparatus. B3GNT2: a major poly-N-acetyllactosamine synthase. GCNT: formation of the core 2 O-glycan branch. FUT1: O-glycan fucosyltransferase. 3OST: sulfotransferase for the 3′-position of galactose. ST3GAL1 and ST3GAL2: O-glycan α2,3-sialyltransferases. ST6GALNAC1, ST6GALNAC2, ST6GALNAC3 and ST6GALNAC4: O-glycan α2,6-sialyltransferases acting on GalNAc as acceptor. AGC: aggrecan. COL2: collagen type-II. COL1: collagen type-I. n.d.: not detectable.