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Table 2 Presence of mRNA for selected glycosyltransferases in OA chondrocytes

From: Glycophenotyping of osteoarthritic cartilage and chondrocytes by RT-qPCR, mass spectrometry, histochemistry with plant/human lectins and lectin localization with a glycoprotein

  

  N-glycans

   

MAN1C1

0.8

MGAT4A

172.6

ST6Gal1

676.8

MAN2A1

449.9

MGAT4B

77.0

ST6Gal2

5.2

MGAT1

723.3

MGAT5A

387.3

ST3Gal3

59.4

MGAT2

2,328.2

MGAT5B

2.9

ST3Gal4

153.7

MGAT3

10.1

FUT8

263.9

ST3Gal6

14.7

  

B4GALNT3

104.4

  
 

  O-glycans

  

  Chondrocyte markers

 

GALNT

3,925.3

ST3Gal1

60.4

AGC

1,704.7

B3GNT

141.8

ST3Gal2

75.7

COL2

9,953.7

GCNT1

375.5

ST6GalNAc1

n.d.

COL1

5,722.9

FUT1

0.4

ST6GalNAc2

n.d.

  

3OST

19.4

ST6GalNAc3

0.7

  
  

ST6GalNAc4

9.8

  
  1. Distinct mRNA species were quantified using RT-qPCR. Numbers denote relative copy numbers with respect to the expression of the GAPDH gene arbitrarily set at 1,000. cDNA of OA chondrocytes from five donors was pooled and analyzed in duplicate. All series of measurements (technical replicates) had a standard deviation below 1.5%.
  2. MAN2A1 and MGAT2: conversion of oligomannosides to complex-type N-glycans. MGAT1: committing step for synthesis of hybrid- and complex-type N-glycans. MAN1C1: trimming of oligomannosidic structures. MGAT3: introduction of bisecting GlcNAc to the core of complex-type glycans in β1,4-linkage. MGAT4 and MGAT5: production of tri- and tetra-antennary N-linked sugar chains. FUT8: transfer of fucose to the core of complex-type glycans in α1,6-linkage. B4GALNT3: β1,4-N-acetylgalactosaminyltransferase 3, forming the LacdiNAc terminus. ST6GAL1, ST6GAL2: N-glycan α2,6-sialyltransferases. ST3GAL3, ST3GAL4, ST3GAL6: N-glycan α2,3-sialyltransferases. GALNT1: initiates O-linked mucin-type glycosylation in the Golgi apparatus. B3GNT2: a major poly-N-acetyllactosamine synthase. GCNT: formation of the core 2 O-glycan branch. FUT1: O-glycan fucosyltransferase. 3OST: sulfotransferase for the 3′-position of galactose. ST3GAL1 and ST3GAL2: O-glycan α2,3-sialyltransferases. ST6GALNAC1, ST6GALNAC2, ST6GALNAC3 and ST6GALNAC4: O-glycan α2,6-sialyltransferases acting on GalNAc as acceptor. AGC: aggrecan. COL2: collagen type-II. COL1: collagen type-I. n.d.: not detectable.