IL-17A dose-responses in dermal fibroblasts. (A) Dermal fibroblasts were cultured in the presence of increasing amounts of IL-17A for 48 hours. Shown are MCP-1, IL-8, MMP-1 and type I collagen levels assessed in fibroblast culture supernatants from three distinct HD squares indicates the mean ± SEM of the protein levels in cultures from three HD in the presence of TNF (1 ng/ml) or TGF-β (10 ng/ml) performed in triplicate. (B) Bars represent the mean ± SEM of the levels of MCP-1, IL-8, MMP-1 and type I collagen assessed in three distinct HD performed in triplicate. Neutralizing anti-IL-17A and irrelevant control ab (ctrl-Ab) were added one hour before IL-17A. Significant differences were assessed by paired Student’s t-test.
HD: healthy donors; IL: interleukin; MCP-1: monocyte chemotactic protein-1; MMP-1: matrix metalloprotein-1; SEM: standard error of the mean; TGF: transforming growth factor; TNF: tumor necrosis factor.