Figure 3

Effect of oxygen tension and FN-f on catabolic and anabolic activities. Chondrocyte/agarose constructs were cultured under uninterrupted experimental conditions with 0 or 1 μM FN-f or 10 ng/ml IL-1β in the presence or absence of 1 mM L-NIO at 1, 5 and 21% oxygen tension for 48 hours, where NO release (A), PGE₂ rowsep="1"production (B) rowsep="1"and GAG synthesis (C). Error bars represent the mean and SEM of 12 values from three separate experiments.