Table 1 Beacon designer sequences and real-time reaction efficiencies of qPCR assays

iNOS

U14640

Probe:5′-FAM- CGCGATC CCTGCTTGGTGGCGAAGATGAGC GATCGCG -

200

DABCYL-3′

200

2.01

Forward: 5′-GTAACAAAGGAGATAGAAACAACAGG-3′

200

(± 0.08)

Reverse: 5′-CAGCTCCGGGCGTCAAAG-3′

Probe: 5′-FAM-

COX-2

AF031698

CGCGATC GTCAGAAATTCGGGTGTGGTACAGTT GATCGCG -DABCYL-3′

200

Forward: 5′-CGAGGTGTATGTATGAGTGTAGG-3′

300

1.99

Reverse: 5′-GTTGGGAGTGGGTTTCAGG-3′=

300

(± 0.04)

Probe: 5′-FAM-

200

Aggrecan

U76615

CGCGATC CACTCAGCGAGTTGTCAGGTTCTGA GATCGCG -DABCYL-3′

2.05

Forward: 5′-TGGTGTTTGTGACTCTGAGG-3′

100

(± 0.07)

Reverse: 5′-GATGAAGTAGCAGGGGATGG-3′

200

Collagen type II

X02420

Probe: 5′-FAM- CGCGAT GCGTCAGGTCAGGTCAGCCAT ATCGCG -

DABCYL-3′

200

Forward: 5′-AAACCCGAACCCAGAACC-3′

100

1.96

Reverse: 5′-AAGTCCGAACTGTGAGAGG-3′

100

(± 0.08)

GAPDH

U85042

Probe: 5′-HEX- CGCGATC CACCATCTTCCAGGAGCGAGATCC GATCGCG -

DABCYL-3′

200

1.96

Forward: 5′-TTCAACGGCACAGTCAAGG-3′

200

(± 0.01)

Reverse: 5′-TTCAACGGCACAGTCAAGG-3′

200