Figure 5

TGF-β1 is essential for BAFF and B cell-induced collagen upregulation. (A,B) Collagen release by SScHDF was determined by the Sircol collagen dye-binding assay in 3-day (A) and 5-day (B) culture supernatants after incubation with anti-μ at 5 μg/ml and rhBAFF at 100 ng/ml in all conditions, and isotype control antibody at 20 μg/ml (white bars), anti-TGF-β1 antibodies at 0.2 μg/ml (dark grey bars), anti-TGF-β1 antibodies at 1 μg/ml (light grey bars), or anti-IL-6 antibodies at 20 μg/ml (black bars). Data are expressed as the median of duplicate samples of three independent experiments ± interquartile range. *P < 0.05.