Table 2 Antibodies used to detect phenotype synovium-derived mesenchymal stem cells (SMSCs) with flow cytometry
Antibody | Fluorochrome | Concentration (μg/ml) | Source |
|---|---|---|---|
CD14 | PE | 200 | Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA) |
CD34 | PE | 50 | Southern Biotech (Birmingham, AL, USA) |
CD44 | FITC | 50 | Becton Dickinson (Bedford, MA, USA) |
CD45 | PE | 10 | Caltag (Burlingame, CA, USA) |
CD90 | PE | 1 | Becton Dickinson (Bedford, MA, USA) |
CD105 | FITC | 10 | Becton Dickinson (Bedford, MA, USA) |
CD166 | FITC | 50 | AbD Serotec (MorphoSys, UK) |
HLA-DR | PE | 200 | Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA) |