Figure 4
IL-29 induces production of Toll-like receptors 2, 3 and 4 in MH7A cells. MH7A cells were stimulated with IL-29 or TNF-α (positive control) for 24 hours, then the expression of Toll-like receptor 2 (TLR2), TLR3 and TLR4 mRNA (A) or protein (B) was measured by real-time PCR or flow cytometry analysis, respectively. (C) Quantitative expression of TLR2, TLR3 and TLR4 protein was summarized. Because the effect of IL-29 at 1 ng/ml on the expression of the above genes in MH7A cells is similar to the medium control group, the data from IL-29 at 1 ng/ml in MH7A are not shown in the figure. The data depicted in (A) and (B) are representative of one of three independent experiments. The error bars represent mean ± SD for triplicate wells. The data presented in (C) are the means ± SD for three independent experiments. *P < 0.05 versus medium control. **P < 0.01 versus medium control.