Upregulation of renal sialoadhesin (Sn) expression following disease onset in BWF1 mice. (A) Renal cortical tissues from 28-week proteinuric BWF1 mice and age -atched NZW mice were examined for gene expression of Sn, CD68 and F4/80 (n = 4 per group) by qRT-PCR. (B) Immunohistochemistry of frozen kidney sections from pre-disease (10 weeks) and diseased (28 weeks) NZBWF1 mice, indicating the anatomical locations of CD68+ and Sn+ macrophages in renal tissues of diseased NZBWF1 mice. (C) Quantification of Sn+ macrophages in glomerular cross-sections (gcs). Statistical analysis was done using the Mann–Whitney test and P-values are indicated.