Figure 1From: NLRP3 promotes autophagy of urate crystals phagocytized by human osteoblastsPhagocytosis of monosodium urate by osteoblasts. Confluent human OBs, previously stained with CellTracker Orange CMTMR, were cultured with vehicle (A) or with 0.5 mg MSU/106 cells (B) for 48 hours at 37°C, and then analyzed with confocal microscopy by using Olympus Fluoview microscope with helium-neon 543-nm lasers (left panel), differential interference contrast (DIC; middle panel), and overlap (right panel); ×400 magnification. Arrows indicate vacuoles and crystals of MSU. Representative of independent experiments with OB from three different donors.Back to article page