Figure 2
From: NLRP3 promotes autophagy of urate crystals phagocytized by human osteoblasts
Effects of MSU on OB viability and proliferation. (A) Confluent OBs were incubated with vehicle (control) or with MSU from 0.3 to 1 mg/106 cells for 24, 48, or 72 hours. Cells were washed with PBS and removed with Accutase. Propidium iodide (PI) exclusion was analyzed with cytofluorometry. (B) Cells were plated 3 days with vehicle or with MSU in 96 wells and then analyzed with the CellTiter 96 AQueous One proliferation assay. Data are represented as a ratio of MSU-stimulated to vehicle-stimulated cells. Results are given as mean ± SEM of values from four (A) or three (B) different donors. Statistical analysis: one-way ANOVA followed by Bonferroni multiple-comparison test: ns, nonsignificant; means without a common letter differ: P < 0.05.