Figure 4

Phosphoproteomic analysis of MSU-stimulated OBs. The Human Phospho-Kinase Array was used to detect multiple phosphorylated kinases in OBs, either untreated or stimulated with 1 mg MSU for 60 minutes. (A) Template showing the location of kinase antibodies spotted onto the Human Phospho-Kinase Array kit. Positive and negative controls are indicated by + and – adjacent to appropriate spots. Signals of relevant kinases in response to MSU stimulation are indicated by numbers. (B, C, D) Quantification of mean spot pixel densities of untreated cells (white) versus MSU-stimulated cells (black). (B) Representation of phosphorylated MAP kinases. (C) Phosphorylated Src kinase family. (D) Phosphorylated TOR and p70S6K. Representative of independent experiments with OB from three different donors. Results are shown as mean ± SEM of densitometric values (n = 3). Statistical analysis was performed by using the paired two-tailed t test to compare phosphorylated proteins from OBs under MSU versus vehicle; *P < 0.05.