NLRP3 promotes autophagy of urate crystals phagocytized by human osteoblasts

Table 1 Effect of pharmacologic inhibitors on MSU phagocytosis by OBs

Confluent OBs were preincubated 15 minutes with 1 μM cytochalasin D or 10 μM colchicine at 37°C and then stimulated with 0.5 mg MSU/10⁶ cells for 24 hours. Confluent OBs were preincubated with PI3K inhibitors: wortmannin 50 nM or LY4294002 10 μM, with PKC inhibitors: GF109203X 5 μM or Gö6976 1 μM, with p38 or ERK1/2 inhibitors: SB203580 10 μM or PD98069 20 μM, or with a Syk inhibitor: piceatannol 20 μM and a Src inhibitor: PP2 10 μM, 15 minutes at 37°C and then stimulated with 0.5 mg MSU/10⁶ cells for 24 hours. Cells were analyzed with microscopy (×200 magnification), and results expressed as cumulative data means of values ± SEM of five pictures for each donor. Statistical analysis was performed on vacuole counts by using the repeated measures ANOVA followed by Tukey multiple-comparison test (n = 4 different donors). *Means without a common letter differ: P < 0.05.

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