Figure 2

IP-10 is identified in patients with localized scleroderma skin specimens, similar to psoriasis. Immunohistochemical (IHC) analysis of IP-10 was performed on localized scleroderma, psoriasis (as positive control) and healthy patient skin specimens. All skin specimens were stained with hematoxylin and eosin (H & E) to visualize cellular infiltrate (A, D, G, J). All specimens were also stained using an IgG control and demonstrated the absence of IP-10 staining for the control (B, E, H, K). There was an absence of IP-10 staining in healthy controls represented by panel C (one illustrated out of two subjects). The psoriasis disease controls demonstrated moderate staining for IP-10, represented by panel F (one illustrated out of two subjects). Localized scleroderma (LS) patients demonstrated moderate IP-10 staining in the dermis, represented by panels I and L (two illustrated out of five subjects). IgG, immunoglobulin G; IP-10, interferon-gamma inducible protein-10.