Table 1 Primers for real-time PCR
From: A complex interaction between Wnt signaling and TNF-α in nucleus pulposus cells
Target | NCBI number | Forward primer, 5'-3' | Reverse primer, 5'-3' |
|---|---|---|---|
β-catenin | AF_121265.1 | GCCAGTGGATTCCGTACTGT | GAGCTTGCTTTCCTGATTGC |
Wnt1 | NM_001105714.1 | TCCTCATGAACCTTCACAATAACGA | TTGCACTCTTGGCGCATCTC |
Wnt3a | NM_001105715.1 | TGTGAGGTGAAGACCTGCTG | AAAGTTGGGGGAGTTCTCGT |
Wnt4 | NM_053402.1 | GAAACGTGCGAGAAGCTCAAAG | AAAGGACTGTGAGAAGGCTACG |
Wnt5a | NM_022631.1 | TGCCACTTGTATCAGGACCA | TGTCTCTCGGCTGCCTATTT |
Wnt5b | NM_001100489.1 | TGACTACTGCCTGCGAAATG | AAAGCAACACCAGTGGAACC |
Wnt6 | NM_001108226.1 | GTCGACTTTGGGGATGAGAA | AAAGCCCATGGCACTTACAC |
Wnt10a | NM_001108227.1 | GTTCCTAGCTCAGGCAGGTG | AAGTCTGTGGAGGGGGAGAT |
Wnt10b | NM_001108111.1 | CTGGTGCTGTTACGTGCTGT | GGAGCCATGATTAACCGAAA |
Wnt16 | NM_001109223.1 | TGGCTGTAACCTCCTCTGCT | GAGGCAATCTCATGCTAGGC |
LRP5 | NM_001106321.2 | CATCCATGCTGTGGAGGA | TGTCTCGGGCACAAGGAT |
LRP6 | NM_001107892.1 | CATGATACGAAGGCACAAGAA | TCTGATTTGGAACCGAGCTT |
LEF1 | NM_130429.1 | TGGTAAACGAGTCCGAAATCA | TGTGTTTGTCCGACCACCT |
TCF4 | NM_053369.1 | TCCAACCCTTCAACTCCTGT | CGTTTCGAGACCAAACAGC |
TNF-α | NM_012675.3 | TGAACTTCGGGGTGATCG | GGGCTTGTCACTCGAGTTTT |
TNFR1 | NM_013091.1 | AATGAGTGCACCCCTTGC | CCTGGGGGTTTGTGACATT |
TNFR2 | NM_130426.4 | GAGGCCCAAGGGTCTCAG | GCTGCCATGGGAAGAATC |