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Figure 8 | Arthritis Research & Therapy

Figure 8

From: Cytokine signaling-1 suppressor is inducible by IL-1beta and inhibits the catabolic effects of IL-1beta in chondrocytes: its implication in the paradoxical joint-protective role of IL-1beta

Figure 8

Degradation of TAK1 via ubiquitination by SOCS1. Representative immunoblots with phosphor-specific antibodies for TAK1 showed no alteration in phosphorylation after treatment with IL-1β in SOCS1-overexpressing SW1353 cells. However, TAK1 expression was reduced in pBABE-SOCS1-transfected SW1353 cells when compared with pBABE-transfected cells (A). Twenty-four hours after transfection with increasing amounts of SOCS1, new protein synthesis was inhibited with 20 μg/ml cycloheximide for 8 hours. TAK1 levels decreased with transient SOCS1 transfection in a dose-dependent manner (B). The level of TAK1 ubiquitination was analyzed via immunoprecipitation with anti-TAK1 and subsequent immunoblotting with anti-ubiquitin antibodies. The amount of ubiquitinated TAK1 protein was higher by SOCS1 overexpression (C). The treatment of MG132 as a proteasome inhibitor dose- and time-dependently increased the levels of TAK1 protein in SOCS1-overexpressing SW1353 cells (D). IP, immunoprecipitation; IB, immunoblotting.

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Arthritis Research & Therapy

ISSN: 1478-6362

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